“Chitosan-based enzyme-immobilized nanocomposite cryogels with controlled biodegradation profile”

Tissue engineering is a technique that applies temporary biomaterials as scaffolds to facilitate the regeneration of native tissue. A critical challenge is precisely controlling the degradation rate of these scaffolds to match the pace of new tissue formation, ensuring optimal integration and functional recovery. In this study, poly(glycerol carbonate methacrylate) (p(GCMA)) nanoparticles with an average diameter of ∼50 nm were successfully synthesized. These nanoparticles were incorporated into chitosan-based nanocomposite cryogels, which exhibited a macroporous structure with pore sizes of 55–60 μm and a swelling capacity 23 to 35 times of their dry weight. Incorporation of p(GCMA) significantly enhanced their flexibility and thermal stability of nanocomposite cryogels. Importantly, p(GCMA)-containing cryogels effectively immobilized up to 7 mg/g of protease within crosslinked network, allowing for sustained …